Antioxidant Activity Correlated with Chlorophyll Pigments and Magnesium Content of Some Green Seaweeds

The complex valorification of seaweed biomass represents a highly important resource for the pharmaceutical and dermo-cosmetic industry, supplying raw material for the extraction of bioactive substances such as, polysaccharides, vitamins, sterols, aminoacids, polyphenols and minerals. Marine algae represent an indefatigability resource of therapeutic active principles for diseases treatment of magnesium deficiency. The presence of chlorophyll pigments in the green algae confirms the magnesium existence, as central element in molecular structures. The main green algae, as Ulva lactuca and Enteromorpha intestinalis present on the Romanian Black Sea Coast, belong to Chlorophyta sp. In this paper we present a study of antioxidant activity correlated with magnesium content and chlorophyll pigments of the green algae Enteromorpha intestinalis and Ulva rigida (syn. Ulva lactuca ) hydroalcoholic extracts. For chlorophyll pigments determination, the UV-Vis spectrometry was used. Magnesium content had been determined by absorption atomic spectrometry method, using HR-CS-AAS ContraA 700 apparatus, Analytik Jena and for total antioxidant capacity of green algae extracts, the photochemiluminescence method had been applied, using Photochem Analytik Jena apparatus. The comparative studies on two marine green algae extracts confirm the high magnesium content correlated with a high level of total antioxidant capacity for


Introduction
Marine algae represent an unequaled nutrients dietary source, [1].The presence of chlorophyll pigments in the green algae confirms the magnesium existence, as central element in molecular structures.The literature mentions that marine green algae are an important resource, a therapeutic bioactive and source of antioxidants principles, such as proteins, oligominerals, vitamins, polyphenols, polysaccharides (e.g.agaragar, alginats), fatty acids, fibers, [2].All of these biocomponents stimulate the endocrine glands, blood circulation and immune system, are alkalinizing, remineralizing, antirheumatic, antiinfectious, antiaging.Their therapeutic action is to improve digestion, detoxify the body, accelerate healing, provide radiation protection, help prevent degenerative diseases and attenuate arthritic pain.Also, the rich content of chlorophyll pigments from green seaweeds, gives them the natural adjuvant status of the body in detoxification and healing processes.Magnesium is a versatile mineral that has some major implications, in the human body ranks fourth in overall abundance, but intracellular it is second only to potassium.Between 60-65% of magnesium in the human body is present in bone.Magnesium that does not exist as part of bone is mainly present within muscle intracellular.About 1% of magnesium is present in the extra cellular fluid.Inside cells, magnesium may be found bound to phospholipids.This mineral is involved in over 300 enzymatic reactions in the body including glycolysis, the Krebs cycle, creatine phosphate formation, nucleic acid synthesis, amino acid activation, cardiac and smooth muscle contractibility, cyclic AMP formation, and protein synthesis.There is a close relationship of reciprocity between the shortage of magnesium and long-states stress, as in the senescence.In contemporary medicine, there is an increased interest regarding magnesium, as this macroelement seems to be involved though various metabolic circuits in the prophylaxis of a large number of diseases, [3 -5, 10 -13].
In this paper, we present a study regarding chlorophyll pigments and magnesium content correlated content with total antioxidant capacity of some green algae, specifics for Romanian Black Sea Coast, belong to Chlorophyta species, such as Cladophora vagabunda (L.) Hoek.and Ulva lactuca Ag. syn.Ulva rigida (L.).
Ulva lactuca (syn.Ulva rigida) is a small genus of marine and brackish water green algae in the Ulvaceae family.It is edible and is often called sea lettuce.The dark to pale green thallus of ulvoid species is flat and blade-like and is composed of two layers of cells.There is no differentiation into tissues; all the cells of the plant are more or less alike except for the basal cells, which are elongated to form attachment rhizoids.Each cell contains one nucleus and has a cup-shaped choroplast with a single pyrenoid.Plants tend to be pale green when young, bright green when mature and dark green when old.Ulva lactuca can be up to 45 cm long and 30 cm across, can be used in cooking, soups, with meats and fish, and salads, [6 -8, 10].
Cladophora vagabunda (L.) Hoek, pale green to grass green alga, present filamentous thallus, spongy, soft tufts, anywhere from 5 -50 cm in length.The plants are small, broom-like in shape and grow to be to 3-4 cm tall.This species grows on rocks in mid to lower intertidal zones along moderately wave exposed shorelines.Are opportunistic settlers that respond to environmental variations, [9 -13].

Material and Methods
The studied algal material were manual harvested from the South Romanian Coast of Black Sea, sector between Mangalia -2 Mai -Vama Veche, the medio-littoral area, in the period July -August 2018, Fig. 1.The green algae biomass was collected in the morning when is a maximum of UV rays, at the temperature between 28°C in July and 25°C in August 2018.Vegetal product was sorted on species, Ulva lactuca Ag. syn.Ulva rigida (L.) and Cladophora vagabunda (L.) Hoek.The immediately pretreatment process after harvesting involves washing the biomass thoroughly several times with distillated water, to remove adhering sand particles and impurities and dried at room temperature, for 24 hours, in the dark.Fresh marine algae biomass was weighed to determine the loss of water through drying process, [14].
The dried algae material was macerated to a fine powder (homogeneous, as well as a higher surface-to-volume ratio) and analyzed for chlorophyll pigments content, magnesium content and total antioxidant capacity, with triplicate samples of each determination.

Macroalgae chlorophylls pigments determination
-Algal thallus was cut into small fragments.0.1 g of each sample was ground in 80% acetone, with sand grains for higher efficiency and incubated in darkness at ambient temperature, for complete extraction.The final extracts in concentration 10% of marine algae, were filtered through a blue band Whatman filter paper.Chlorophyll pigments content by UV-Vis Spectrophotometric method, at specific wavelengths 647 nm and 663 nm, using a WPA S106 spectrometer were determined, with 80% acetone as blank.The concentrations of chlorophyll a and chlorophyll b were calculated by using specific equations according to Lichthenthaler and Buschmann .Subsequently, the amount of chlorophyll/g of algal material was calculated, [15,16,17].

Macroalgae magnesium content determination
-Sample preparation.Solid samples were dried until 105°C, in order to reach constant mass.For mineralization after decantation, samples were filtered on Whatman quantitative filter paper.After drying, marine biomass algae samples were mineralized with concentrated acids in order to determine the presence and concentration of metallic elements, at controlled temperature and pressure in the digestion system.After finishing this process, the content of the digestion vessels was introduces in 25 mL marked flacks and brought to volume with twice-distilled and deionized water.The controls for the AAS methods were: 1 sample of concentrated acids (with variable volumes, depending on the type of analysed sample), subjected to digestion process, consisting in the following mixture: 2 mL H2SO4 96%, 2 mL H3PO4 85%, 2 mL HF 40% and 1 mL HNO3 65%.
-Concentrations of magnesium were measured using atomic absorption spectrometry methods.All the materials foreseen for these analytical determinations comply with the quality standards in force.Apparatus: High Resolution Continuum Source Atomic Absorption Spectrometer ContrAA 700, Analytik Jena AG, Germany, with autosampler for dilution sample, on acetylene flame technique, sequential analysis, at specific wavelengths 285.2125 nm.Furnace parameters: Pyrolysis Temperature: 850 °C; Atomization Temperature: 1500 °C; Samples and standards were diluted with ultrapure deionized water, [15,18,19].

Macroalgae total antioxidant capacity determination
-Sample preparation.To determine total antioxidant capacity of the vegetal samples, the stock solution was prepared as follows: well-dried algae were ground, triturated to fine powder, then a cold extract of each species of algae was got, using 1g and respectively 10 g of dry vegetable raw material (algal species), which were placed in volumetric flasks (100 mL) and made up to the mark with 70% ethylic alcohol.Extraction was performed for 12 days, at room temperature, at darkness.Mixtures were shaken regularly and separation of extracts was done by decanting, then were filtered under normal pressure on a blue band Whatman filter paper.Resulted 1% and 10% concentration of fluid extracts, herein after referred to as clear, with specific algae odor and color depending on species.Before to the measurement, the samples were rapidly homogenized using a Vortex Velp Scientifica, Italy agitator and 10 μL volume of the sample were taken from the supernatant.Each determination lasted 120 seconds, [18,21,22].
Exposure to external radiation from a Hg lamp lined with phosphor that provides the maximum energy at λ = 351 nm, photosensitive reagent, produces free radicals in the sample for analysis, resulting a photochemical reaction.The free radicals (superoxide anion radicals) produced by optical excitation of a photosensitized substance added in standardized volumes, are partially eliminated by reaction with the antioxidants present in the sample.The residual radicals cause the detector substance to luminescence, which is then exactly determined in a separate cell by means of a photomultiplier tube.The measuring signal produced by the luminescence is traced over 3 min.The total antioxidant capacity of the sample is measured by converting the electrical signal which is then converted into concentration values and is quantified by comparison with the standard Trolox® expressed as Trolox equivalency nmol/sample, [21 -29].
From stock solution were prepared the samples, as follow (Table 1):

Magnesium content determination
-The results obtained emphasize that magnesium content was increased for both algae species.
-For Cladophora vagabunda L. species, a higher content of magnesium (56 mg/L) compared to that presents in Ulva lactuca L. species (48 mg/L), at the same extracts concentration10% algae, was registered.

Total antioxidant capacity (TEAC)
-Total antioxidant capacity (TEAC) of algae fluid extracts was reported according to the ACL procedure, quantified by comparison with standard substance Trolox.The results are expressed in nmol/vol.sample, Trolox equivalent units, according to Table 2.The obtained experimental results highlight the following aspects: -The extraction method used to obtain fluid extracts, cold maceration in 70% ethyl alcohol for 12 days in the dark, generated an increased total antioxidant capacity.
-At concentration 10% of the extracts, both algae species present an increased total antioxidant capacity.-Ulva lactuca algae samples emphasize the lowest antioxidant capacity at 1%, respectively 10% concentrations of the extracts.
-The behavior observed for the two studies seaweeds, could be correlated with the highest content in magnesium and chlorophylls of Cladophora vagabunda species compared with decreased content for Ulva lactuca species.The obtained values are in accordance with literature [21,22].

Conclusion
Regarding the two studied green seaweeds species present along South Romanian Black Sea Coast, Cladophora vagabunda (L.) Hoek.registered an increased values of chlorophyll pigments and magnesium content compared to those for Ulva lactuca (L.);Also, both algae species present a high antioxidant activity, most intensive being at the Cladophora vagabunda (L.) Hoek.The results obtained emphasize the possibility of opening new directions in the process of complex valorification of these bioresources offered by the Black Sea, for obtaining new pharmaceutical and dermato-cosmetic products from available natural resources of Romanian littoral.

Fig. 1 .
Fig. 1. Green seaweed biomass along South Romanian Black Sea Coast

Fig. 3 . 4 .-
Fig. 3. Calibration curve for standard Trolox (ACL method, Analytik Jena AG) Results and Discussions Macroalgae pigments content determination Chlorophylls pigments contents of green algae fluid extracts from Mangalia -2 Mai -Vama Veche area, at 10% concentration of algal extracts, emphasize an increased values.Chlorophyll a and chlorophyll b content presented high values for both species, Fig. 4. -For Cladophora vagabunda L. Hoek high value of chlorophyll a (59.88207 mg/L) compared to Ulva lactuca L. (16.3455 mg/L) was registered, respectively for Cladophora vagabunda L. Hoek high value of chlorophyll b (48.24553 mg/L) compared to Ulva lactuca L. (8.595833 mg/L) was registered.